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《医学前沿(英文)》 2016年 第10卷 第1期 页码 61-75 doi: 10.1007/s11684-016-0436-0
The latent expression pattern of Epstein-Barr Virus (EBV) genes in nasopharyngeal carcinoma (NPC) has been extensively investigated, and the expression of several lytic genes in NPC has been reported. However, comprehensive information through EBV transcriptome analysis in NPC is limited. We performed paired-end RNA-seq to systematically and comprehensively characterize the expression of EBV genes in NPC tissue and C666-1 NPC cell line, which consistently carries EBV. In addition to the transcripts restricted to type II latency infection, the type III latency EBNA3s genes and a substantial number of lytic genes, such as BZLF1, BRLF1, and BMRF1, were detected through RNA-seq and were further verified in C666-1 cells and NPC tissue through real-time PCR. We also performed clustering analysis to classify NPC patient groups in terms of EBV gene expression, which presented two subtypes of NPC samples. Results revealed interesting patterns of EBV gene expression in NPC patients. This clustering was correlated with many signaling pathways, such as those related to heterotrimeric G-protein signaling, inflammation mediated by chemokine and cytokine signaling, ribosomes, protein metabolism, influenza infection, and ECM-receptor interaction. Our combined findings suggested that the expression of EBV genes in NPC is restricted not only to type II latency genes but also to type III latency and lytic genes. This study provided further insights into the potential role of EBV in the development of NPC.
关键词: Epstein-Barr virus paired-end transcriptome sequencing latency genes lytic genes nasopharyngeal carcinoma
Metagenomic analysis on resistance genes in water and microplastics from a mariculture system
《环境科学与工程前沿(英文)》 2022年 第16卷 第1期 页码 4-4 doi: 10.1007/s11783-021-1438-y
Microplastics existing widely in different matrices have been regarded as a reservoir for emerging contaminants. Mariculture systems have been observed to host microplastics and antibiotic resistance genes (ARGs). However, more information on proliferation of ARGs and metal resistance genes (MRGs) in mariculture system at the presence of microplastics is needed.
关键词: Antibiotic resistance genes Metal resistance genes Metagenomic analysis Microplastics Mariculture
Mengli Wang, Ruying Li, Qing Zhao
《环境科学与工程前沿(英文)》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1127-2
Sludge digestion is critical to control the spread of ARGs from wastewater to soil. Fate of ARGs in three pretreatment-AD processes was investigated. UP was more efficient for ARGs removal than AP and THP in pretreatment-AD process. The total ARGs concentration showed significant correlation with 16S rRNA gene. The bacteria carrying ARGs could be mainly affiliated with Proteobacteria.
关键词: Tetracycline resistance genes Sulfonamide resistance genes 16S rRNA gene Sludge pretreatment Anaerobic sludge digestion
《环境科学与工程前沿(英文)》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1415-5
• ARGs were detected in livestock manure, sludge, food waste and fermentation dregs.
关键词: Antibiotic resistance genes Organic solid waste Aerobic composting Livestock manure Sludge
《环境科学与工程前沿(英文)》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1521-z
• UV/chlorine can effectively remove VBNC pathogens, ARGs and MGEs in reclaimed water.
关键词: UV/chlorine process Pathogen Antibiotic resistance genes High-throughput qPCR Reclaimed water
《环境科学与工程前沿(英文)》 2022年 第16卷 第3期 doi: 10.1007/s11783-021-1469-4
• Distribution of ARGs in decentralized sewage facilities were investigated.
关键词: Decentralized sewage treatment facilities Antibiotic resistance genes Virus Metagenomics Network analysis
Research progress in pathogenic genes of hereditary non-syndromic mid-frequency deafness
null
《医学前沿(英文)》 2016年 第10卷 第2期 页码 137-142 doi: 10.1007/s11684-016-0449-8
Hearing impairment is considered as the most prevalent impairment worldwide. Almost 600 million people in the world suffer from mild or moderate hearing impairment, an estimated 10% of the human population. Genetic factors play an important role in the pathogenesis of this disorder. Hereditary hearing loss is divided into syndromic hearing loss (associated with other anomalies) and non-syndromic hearing loss (not associated with other anomalies). Approximately 80% of genetic deafness is non-syndromic. On the basis of the frequency of hearing loss, hereditary non-syndromic hearing loss can be divided into high-, mid-, low-, and total-frequency hearing loss. An audiometric finding of mid-frequency sensorineural hearing loss, or a “bowl-shaped” audiogram, is uncommon. Up to now, merely 7 loci have been linked to mid-frequency hearing loss. Only four genetic mid-frequency deafness genes, namely, DFNA10 (EYA4), DFNA8/12 (TECTA), DFNA13 (COL11A2), DFNA44 (CCDC50), have been reported to date. This review summarizes the research progress of the four genes to draw attention to mid-frequency deafness genes.
关键词: hereditary non-syndromic hearing loss mid-frequency hearing loss deafness genes
《环境科学与工程前沿(英文)》 2022年 第16卷 第7期 doi: 10.1007/s11783-021-1499-y
• Manure fertilization resulted in antibiotic residues and increased metal contents.
关键词: Pig manure Antibiotics Metals Antibiotic resistance genes Paddy fields
Effects of dwarfing genes on water use efficiency of bread wheat
Jiakun YAN, Suiqi ZHANG
《农业科学与工程前沿(英文)》 2017年 第4卷 第2期 页码 126-134 doi: 10.15302/J-FASE-2017134
关键词: coleoptile length wheat dwarfing genes grain yield root water use efficiency
Menglu Zhang, Sheng Chen, Xin Yu, Peter Vikesland, Amy Pruden
《环境科学与工程前沿(英文)》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1124-5
Extracellular DNA structure damaged by chlorination was characterized. Integrity of extracellular ARG genetic information after chlorination was determined. Typical chlorine doses will likely effectively diminish extracellular DNA and ARGs. Plasmid DNA/ARGs were less readily broken down than genomic DNA. The Bioanalyzer methodology effectively documented damage incurred to DNA.
关键词: Antibiotic resistance Antibiotic resistance genes (ARGs) Extracellular DNA/ARGs Chlorination
Design of tandem genes cluster for isoflavone engineering
Xunli XIA PhD , Guangxiao YANG PhD , Guangyuan HE PhD ,
《医学前沿(英文)》 2009年 第3卷 第3期 页码 292-296 doi: 10.1007/s11684-009-0057-y
关键词: isoflavones engineering secondary metabolism tandem genes cluster
《环境科学与工程前沿(英文)》 2021年 第15卷 第5期 doi: 10.1007/s11783-020-1342-x
• SMX addition had negative effect on acetoclastic methanogens in mesophilic AD.
关键词: Pig manure Antibiotics Anaerobic digestion Resistance genes Microbial community
Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere
Lu Song, Can Wang, Yizhu Wang
《环境科学与工程前沿(英文)》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1274-5
关键词: Airborne tetracycline resistance genes Filter sampling Capture loading Membrane pretreatment Indoor laboratory atmosphere
《环境科学与工程前沿(英文)》 2023年 第17卷 第7期 doi: 10.1007/s11783-023-1682-4
● Abundance of MAGs carrying ARG-VF pairs unchanged in rivers after WWTP upgrade.
关键词: Wastewater treatment plant upgrade Antibiotic resistance genes (ARGs) Virulence factors (VFs) Gene co-occurrence Genome-centric analysis
Lu Song, Can Wang, Yizhu Wang
《环境科学与工程前沿(英文)》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1289-y
标题 作者 时间 类型 操作
Comprehensive profiling of EBV gene expression in nasopharyngeal carcinoma through paired-end transcriptome sequencing
null
期刊论文
Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline
Mengli Wang, Ruying Li, Qing Zhao
期刊论文
Environmental effects and risk control of antibiotic resistance genes in the organic solid waste aerobic
期刊论文
ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes
期刊论文
Distribution of antibiotic resistance genes and their association with bacteria and viruses in decentralized
期刊论文
Antibiotic resistance genes in manure-amended paddy soils across eastern China: Occurrence and influencing
期刊论文
Degradation of extracellular genomic, plasmid DNA and specific antibiotic resistance genes by chlorination
Menglu Zhang, Sheng Chen, Xin Yu, Peter Vikesland, Amy Pruden
期刊论文
Design of tandem genes cluster for isoflavone engineering
Xunli XIA PhD , Guangxiao YANG PhD , Guangyuan HE PhD ,
期刊论文
with sulfamethoxazole and norfloxacin: Dynamics of microbial communities and evolution of resistance genes
期刊论文
Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere
Lu Song, Can Wang, Yizhu Wang
期刊论文
Mitigating microbiological risks of potential pathogens carrying antibiotic resistance genes and virulence
期刊论文